Cell-to-Cell Adhesion and Electrical Parameters of Murine Embryo Membranes After Cryopreservation by Vitrification

In this work, we have studied the impact of various stages cryopreservation by vitrification in ethylene glycolsucrose medium on plasma membranes adhesive properties 8-cell murine embryos and their specific electrical conductivity. Embryos were divided into following experimental groups: control, group 1 (incubation medium) 2 (complete cycle cryopreservation). The embryo exposure to did not affect ability cell-to-cell adhesion. After a complete cryopreservation, no adhesion was observed. Electrical conductivity determined using pulsed conductometry. incubation medium, resistance electric pulse shown decrease, that manifested phenomenon irreversible breakdown. average values varied within ranges: ((12.1 ± 1.5)...(55.5 2.6)), ((28.7 5.7)...(44.± 8.9)), ((31.0 9.3)...(87.9 26.1)) μS/cm groups 2, respectively. These findings may be explained appearance first structural disorders lipid bilayer even at stage medium. lack blastomeres after vitrification-warming testified damage membrane proteins.